Brand

MultiSciences

CatNum

70-EK1201

ProductName

Human Transferrin/TF ELISA Kit

CustomsName

Human Transferrin/TF ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened) standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

50 l (prediluted)

Sensitivity

2.82 pg/ml

Standard Curve Range

937.50 - 60000 pg/ml

Spike Recovery Range

-

Mean Spike Recovery

-

CV of Intra plate

1.8 % - 2.6 %

CV of Inter plate

1.9 % - 3.7 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against TF
Human TF Standard, lyophilized
TF Detect Antibody
Assay Buffer (10)
Substrate (TMB)
Stop Solution
Washing Buffer (20)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TF. The Human Transferrin /TF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Transferrin (TF) is a secreted protein which belongs to the transferrin family. It is expressed by the liver and secreted in plasma. TF has a molecular weight of around 80 KDa and contains two specific high-affinity Fe (III) binding sites. TFs are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. The main role is to deliver iron from absorption centers in the duodenum and white blood cell macrophages to all tissues.
TF is associated with the innate immune system. The level of TF decreases in inflammation. An increased plasma TF level is often seen in patients suffering from iron deficiency anemia. A decreased plasma transferrin can occur in iron overload diseases and protein malnutrition. Most recently, TF and its receptor have been shown to diminish tumour cells when the receptor is used to attract antibodies. TF is an acute phase protein and is therefore seen to decrease in inflammation, cancers and certain diseases.