Brand

MultiSciences

Cat Num

70-EK1169

Product Name

Human LMAN2/VIP36 ELISA Kit

Customs Name

Human LMAN2/VIP36 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened)standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

100 l (prediluted)

Sensitivity

7.26 pg/ml

Standard Curve Range

125.00 - 8000 pg/ml

Spike Recovery Range

74% - 103%

Mean Spike Recovery

0.84

CV of Intra plate

5.5 % - 6.4%

CV of Inter plate

6.3 % - 7.6 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against LMAN2
Human LMAN2 Standard, lyophilized
LMAN2 Detect Antibody
Assay Buffer (10)
Substrate (TMB)
Stop Solution
Washing Buffer (20)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LMAN2. The Human LMAN2/VIP36 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
LMAN2 (Lectin mannose-binding 2), also known as VIP36, is a protein that in humans is encoded by the LMAN2 gene. LMAN2 has been originally isolated from MDCK cells as a component of glycolipid-enriched detergent-insoluble complexes containing apical marker proteins. LMAN2 binds glycoproteins that retain the intact D1 mannosyl branch in the cis-Golgi network and recycles to the endoplasmic reticulum where, due to higher pH, it releases its cargos, thereby contributing to the quality control of glycoproteins. LMAN2 is an intracellular lectin that cycles between the endoplasmic reticulum and the Golgi apparatus, and is thought to act as a cargo receptor in the transport and sorting of glycoproteins. The interaction between recombinant soluble LMAN2 and BiP is dependent on divalent cations but not on ATP.