Brand

MultiSciences

70-EK103

Product Name

Human IL-3 ELISA Kit

Customs Name

Human IL-3 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened) standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

50 l

Sensitivity

0.94 pg/ml

Standard Curve Range

15.63 - 1000 pg/ml

Spike Recovery Range

87 % - 119 %

Mean Spike Recovery

1.01

CV of Intra plate

4.3 % - 9.5 %

CV of Inter plate

1.5 % - 8.3 %

Release Date

Components

96-well polystyrene microplate coated with a monoclonal antibody against IL-3

Human IL-3 Standard, lyophilized

IL-3 Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10)

Substrate (TMB)

Stop Solution

Washing Buffer (20)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-3. The Human IL-3 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin 3 (IL-3) encoded by the IL3 gene is a cytokine secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., erythropoietin (EPO), granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6.